Phage-typing is the most widely recognised typing method for Staphylococcus aureus (Parker, 1972), and is also still used widely for sub-dividing serotypes of P. aeruginosa (Bergan, 1978) and Salmonella / Shigella spp.

Which of the following bacteria can be typed by phage typing method?

Phage-typing is the most widely recognised typing method for Staphylococcus aureus (Parker, 1972), and is also still used widely for sub-dividing serotypes of P. aeruginosa (Bergan, 1978) and Salmonella / Shigella spp.

What are the 3 types of phages?

Phages can also be categorized into three types according to their infection mechanism: (1) virulent phages always lyse the infected bacterial cell to release their progeny; (2) temperate phages can either enter the lytic cycle as virulent phages or enter the lysogenic cycle in which the phage genome is retained as a …

What is a beta prophage?

A prophage is a bacteriophage (often shortened to “phage”) genome that is integrated into the circular bacterial chromosome or exists as an extrachromosomal plasmid within the bacterial cell.

What type of phage is used in phage typing?

Phage Isolation Phages used for phage typing are generally isolated from the native habitats of the host bacterial strain. These may include sewage, feces, soil, and water. Temperate phages may be isolated from the bacterium itself since it is incorporated into the bacterial genome during lysogenization.

What is phage typing quizlet?

Phage typing. A way of separating different strains of bacteria based on their susceptibility to different viruses.

What is the purpose of phage typing quizlet?

Phage typing is a method used for detecting single strains of bacteria. It is used to trace the source of outbreaks of infections.

What are bacteriophages types?

Phages are classified in a number of virus families; some examples include Inoviridae, Microviridae, Rudiviridae, and Tectiviridae. Like all viruses, phages are simple organisms that consist of a core of genetic material (nucleic acid) surrounded by a protein capsid.

What are two types of phages?

There are two primary types of bacteriophages: lytic bacteriophages and temperate bacteriophages. Bacteriophages that replicate through the lytic life cycle are called lytic bacteriophages, and are so named because they lyse the host bacterium as a normal part of their life cycle.

What is prophage or temperate phage?

Temperate phage: Phage which can undergo either virion-productive or lysogenic cycles. Prophage: Phage genome that replicates with its host cell while not generating virion progeny. Cryptic prophage: Prophage that has mutationally lost its ability to enter a virion-productive cycle.

Is M13 a temperate phage?

Phages are divided into three main classes based on their production and generation: lytic phages such as T4; temperate phages like Lambda; and lysogenic phages such as M13.

What information does phage typing provide?

Phage typing is still used to identify and distinguish different strains within a given species when isolated from different origins (disease, food, water, environmental) or geographical locations.

What valuable information does phage typing provide?

What is the basis of phage typing?

Lenie Dijkshoorn, Kevin Towner, in New Approaches for the Generation and Analysis of Microbial Typing Data, 2001 The basis of phage-typing is the variable sensitivity of isolates to defined collections of bacteriophages which have been selected to provide the maximum sensitivity for differentiating isolates within a particular species.

What are the different types of phages?

Depending on the method of replication phages can be broadly classified as virulent phages (replicate via lytic pathway) and temperate phage (replicate via both lytic and lysogenic pathway). In phage typing, a panel of lytic phages is inoculated on a lawn inoculum of the bacteria under investigation.

What are bacteriophages?

Bacteriophages, also known as phages, are viruses that attack bacteria. Depending on the method of replication phages can be broadly classified as virulent phages (replicate via lytic pathway) and temperate phage (replicate via both lytic and lysogenic pathway).

What is phage typing for Salmonella?

Phage typing is also done for other species of Salmonella e.g. Salmonella Enteritidis PT4. Similarly, Staphylococci are typed to determine whether the isolates belonged to the more virulent phage types so that the appropriate infection control method could be instituted.