Neisseria gonorrhoeae is one of the causatives of sexually transmitted diseases, and it is a fastidious organism. This organism is typically cultured using an agar medium such as chocolate agar plate (GCII agar base with 1% IsoVitaleX [BBL] and purified hemoglobin).

What media is used to grow gonorrhea?

Neisseria gonorrhoeae is one of the causatives of sexually transmitted diseases, and it is a fastidious organism. This organism is typically cultured using an agar medium such as chocolate agar plate (GCII agar base with 1% IsoVitaleX [BBL] and purified hemoglobin).

How does Neisseria gonorrhoeae grow on chocolate agar?

5 Neisseria gonorrhoeae on Chocolate Agar. Plates are incubated in a CO2-enriched, humid atmosphere (some gonococci require CO2 for growth, the growth of all species is enhanced by carbon dioxide). Colonies of N. gonorrhoeae vary in diameter from 1 to 4,0 mm after 48 hours.

Which media is used for isolation of gonorrhea?

GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens.

Does Neisseria gonorrhoeae grow on blood agar?

Neisseria gonorrhoeae is the causative agent of gonococcal infections. Gonococci are not able to grow on common blood agar. Most Neisseria strains have complex growth requirements. Some strains may be exquisitely sensitive to fatty acids, necessitating the incorporation of soluble starch in the growth media.

What type of media is Martin Lewis agar?

Martin Lewis Agar is a selective and enriched medium for the isolation and cultivation of Neisseria species from mixed flora. Hemoglobin, Bio-X, and dextrose incorporated in the culture media provide enough nutrient agents to permit luxuriant growth of fastidious microorganisms.

Is Thayer-Martin agar enriched?

It is a selective and enriched medium for the isolation and cultivation of Neisseria sp. from mixed flora with suppression of most other gram-negative diplococci, gram-negative bacilli, gram-positive organisms, and yeast.

Does Neisseria grow on MacConkey?

Note: Neisseria does not grow on MacConkey. Note: Growth, but no fermentation of lactose.

Does Haemophilus influenzae grow on MacConkey?

Haemophilus spp. do not grow on MacConkey agar. Haemophilus influenzae has been shown to cause invasive infection such as meningitis and there is direct spread from water droplets from the upper respiratory tract of infected individuals. The development of the H.

How do you grow N. gonorrhoeae?

A fastidious organism, N. gonorrhoeae requires enriched media in a CO2 atmosphere at 35 degrees to 37 degrees C for growth. In addition, N. gonorrhoeae expresses potent autolysins whose activity increases following glucose depletion during stationary phase, leading to cell death.

Can gonorrhea be cultured?

Gonorrhea cultures are incubated for a minimum of 48-72 hours. Growth on MTM is screened for presumptive positives by oxidase test and gram stain. Positive cultures are confirmed by biochemical identification.

Can Neisseria gonorrhoeae grow on Mac?

Note: Neisseria does not grow on MacConkey.

Is Neisseria gonorrhoeae beta hemolytic?

Group A beta-hemolytic streptococci (GABHS) are responsible for most bacterial cases of acute pharyngotonsillitis, although other pathogens, such as Neisseria gonorrhoeae, Arcanobacterium haemolyticum, Mycoplasma pneumoniae, and Chlamydia pneumoniae, may be the causative agents in sporadic cases.

What is the best Culture Media for Caco-2 cells?

Caco-2 cells are very easy to culture. The standard medium for cell proliferation is DMEM/F12 supplemented with 10% (v/v) FCS and antibiotics. Cell are growing in a C02 incubator (37°C 5% Co2). Depending on cell density, the media is changed every 2-3 days and cells are detached from plastic support by using trypsin/EDTA solution.

What are the conditions required for Caco-2 cell culture?

Normally cultures kept at 37°C in a 5% CO2/95% air atmosphere. Caco-2 cells are mostly used as a confluent monolayer on a cell culture inserts filters. The Caco-2 monolayer is widely used across the pharmaceutical industry as an in vitro model of human small intestinal mucosa to predict the absorption of orally administered drugs.

What is the Caco-2 subculture protocol?

This protocol differs from standard protocols mainly because Caco-2 cells are subcultured when they reach just 50% of confluence, instead of 80%, retaining a high proliferation potential. When this cell population is seeded at high density on filter inserts differentiates almost synchronously and much more homogenously.

Can Caco-2 cells be used as a model for drug absorption?

Caco-2 cells have been of great utility in intestinal absorption studies due to their exclusive ability to model human absorption characteristics and Caco-2 is the most commonly used intestinal permeability model. Caco-2 cells and intestinal enterocytes cells have enabled the validation of the application of Caco-2 cells in drug absorption studies.