Loeffler Serum Medium Base is used for the cultivation of corynebacterial. Due to the serum content, this medium can be used for proteolytic activity and enzymatic hydrolysis of proteins. The gray-white surface is an exceptional background for detection of the microorganism’s pigmentation.

What is Loeffler serum medium?

Loeffler Serum Medium Base is used for the cultivation of corynebacterial. Due to the serum content, this medium can be used for proteolytic activity and enzymatic hydrolysis of proteins. The gray-white surface is an exceptional background for detection of the microorganism’s pigmentation.

Is Loeffler medium selective?

It is recommended that biochemical, immunological, molecular, or mass spectrometry testing be performed on colonies from pure culture for complete identification. It is recommended that selective and non-selective media be inoculated in parallel to Loeffler Medium for isolating C.

What type of media is Loeffler serum slope?

Remel Loeffler’s Medium is a solid medium recommended for use in qualitative procedures for the cultivation of Corynebacterium diphtheriae from clinical specimens. In 1887, Loeffler developed a medium containing horse serum, beef heart infusion, and dextrose for cultivating corynebacteria. 1 Buck and Perry et al.

What is Loeffler’s Agar?

Due to its serum content, Loeffler medium can be used for the determination of proteolytic activities of microorganisms. The gray-white surface of the medium provides an excellent background for the detection and observation of colonial pigmentation.

What is example of enriched media?

Enrichment media allow the growth of a particular type of microorganism in the medium. The examples of enriched media are blood agar, chocolate agar, Loeffler’s serum, etc. The examples of enrichment media are Selenite F broth, tetrathionate broth, alkaline peptone water (APW), etc.

What is a common medium used to culture microorganisms?

The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or lysogeny broth medium. Liquid media are often mixed with agar and poured via a sterile media dispenser into Petri dishes to solidify. These agar plates provide a solid medium on which microbes may be cultured.

What is enriched media in microbiology?

Enriched media refers to the media which contain the nutrients required to support the growth of a wide variety of organisms, including some fastidious ones. Enrichment media refers to the liquid media that inhibits the growth of unwanted bacteria. Enriched media allow the growth of a wide variety of microorganisms.

What are metachromatic granules?

Volutin granules (metachromatic or Babes-Ernst granules) are highly refractive, strongly basophilic bodies consisting of polymetaphosphate. They appear reddish when stained with polychrome methylene blue or toluidine blue (metachromasia).

What is enrichment media in microbiology?

Enrichment media refers to the liquid media that inhibits the growth of unwanted bacteria. Enriched media allow the growth of a wide variety of microorganisms. Enrichment media allow the growth of a particular type of microorganism in the medium.

What is an enriched medium?

Enriched media refers to the media which contain the nutrients required to support the growth of a wide variety of organisms, including some fastidious ones. Enrichment media refers to the liquid media that inhibits the growth of unwanted bacteria.

What is differential medium?

Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups.

What is the Loeffler medium?

Loeffler Medium is a modification of the original formula developed by Loeffler in 1887. Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms, especially from nose and throat.

Which microorganisms produce metachromatic granules on Loeffler medium?

Gram-positive microorganisms other than Corynebacterium may produce metachromatic granules when grown on Loeffler Medium. Detection of proteolysis by some microorganisms may require incubation periods beyond the recommended four days. Read Also

What does Corynebacterium look like on Loeffler medium?

Observe for typical colonial morphology. Growth of Corynebacterium species on Loeffler Medium appears as Corynebacterium species reveal metachromatic granules and appearance suggestive of Chinese-letter formation in methylene blue stain.