GAL80 encodes a transcriptional repressor involved in transcriptional regulation in response to galactose (2).

Is GAL80 a repressor protein?

GAL80 encodes a transcriptional repressor involved in transcriptional regulation in response to galactose (2).

What does GAL80 bind to?

The Saccharomyces cerevisiae Gal80 protein has two binding partners: Gal4 and Gal3. In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, preventing GAL gene expression.

What is GAL80 gene?

The Saccharomyces cerevisiae GAL80 gene ( SGDID:S000004515 ) is a negative regulator of the Saccharomyces cerevisiae GAL4 transcriptional activator. A dimer of GAL80 binds to a GAL4 dimer, such that although GAL4 can still bind the UAS sequence it can no longer activate transcription (adapted from FBrf0233764).

How does Gal80 inhibit Gal4?

In the absence of galactose, the Gal80 protein binds to a small peptide (amino acids [aa] 855 to 870) within the Gal4AD and thereby prevents Gal4-mediated promoter activation (10, 11). Galactose converts Gal3 to a form that readily binds to Gal80 (21).

What is Gal4p?

Gal4p mediates expression of most of the genes involved in utilization of galactose, a relatively poor carbon source that is used only if glucose or other carbon sources are not available.

What is the consequence of DNA methylation?

DNA methylation, a process of adding a methyl group to DNA done by a DNA methyltransferase is a heritable (epigenetic) alteration leading to cancer, atherosclerosis, nervous disorders (Imprinting disorders), and cardiovascular diseases.

What does it mean to be constitutively expressed?

Definition. Constitutive expression means the continuous transcription of a gene in an organism.

How does GAL80 inhibit Gal4?

What is the effect of GAL80 binding on GAL4 function quizlet?

-in absence of galactose, GAL80 binds GAL4 w/ high affinity preventing transcriptional activation. -in presence of galactose, GAL3-galactose complex binds GAL80 to release it from GAL4 allowing transcriptional activation.

How can Micrornas Mirnas regulate gene expression?

microRNA controls gene expression mainly by binding with messenger RNA (mRNA) in the cell cytoplasm. Instead of being translated quickly into a protein, the marked mRNA will be either destroyed and its components recycled, or it will be preserved and translated later.

What is the role of gal80p?

In conjunction with Gal4p and Gal3p, Gal80p coordinately regulates the expression of genes containing a GAL upstream activation site in their promoter (UAS-GAL), which includes the GAL metabolic genes GAL1, GAL10, GAL2, and GAL7 (reviewed in 3 ). Cells null for gal80 constitutively express GAL genes, even in non-inducing media ( 4 ).

How is the GAL80 gene regulated?

GAL80 transcription is also regulated by the Gal3p/Gal80p/Gal4p feedback mechanism that regulates the other GAL genes.

What is the difference between Gal4 and GAL80?

Gal4 binds to GAL gene UASGAL (upstream activation sequence in GAL gene promoter) sites as a dimer via its N-terminal domain and activates transcription via a C-terminal transcription activation domain (AD). In the absence of galactose, a Gal80 dimer binds to a dimer of Gal4, masking the Gal4AD.

What is GAL80 deletion by CRISPR-Cas9 in engineered Saccharomyces cerevisiae?

The gal80 Deletion by CRISPR-Cas9 in Engineered Saccharomyces cerevisiae Produces Artemisinic Acid Without Galactose Induction