Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter.

How do you prepare ampicillin?

Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter.

How is ampicillin antibiotic stock solution prepared?

Preparation of 80ml stock solution Weigh 4g of ampicillin into a small weigh boat. Add 80ml of milliQ to a 250ml bottle. Mix/vortex so all the ampicillin goes into solution. Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.

How do you make 100 ug mL ampicillin?

For example: If you’ll be preparing plates with a final concentration of 100 ug/mL ampicillin, you should make a stock solution of 100,000 ug/mL (100 mg/mL). Simply measure out 100 mg of ampicillin powder, add it to 1 mL of water, dissolve by vortexing, and filter sterilize.

How do you make 1000x ampicillin stock?

1) Dissolve 1 g of ampicillin into 10 ml of ddH2O. 2) Filter through a 0.22 µm filter to sterilize. 3) Aliquot and store at -20°C. 4) Use at 1:1000 dilution in LB or LB-Agar.

How do you make 50 ug ml ampicillin?

Ampicillin – The frozen stock solutions of ampicillin are at 50 mg/ml and 100 mg/ml in H2O, and are marked with a red sticker. The final concentration for LB liquid culture is 50 ug/ml. To obtain this in 100ml (the amount in each LB bottle), add 100ul of 50 mg/ml stock solution OR 50ul of 100 mg/ml stock solution.

How do you dilute ampicillin?

Add 1.5 ml water for injections to 500mg vial contents. Dissolve 500 mg in 10 ml water for injections. Administer by slow injection (three to four minutes). Ampicillin may also be added to infusion fluids or injected, suitably diluted, into the drip tube over a period of three to four minutes.

How do you dissolve ampicillin in water?

To get past 100mM of anhydrous ampicillin dissolved you would need to lower the pH to ~3.44 in 70:30 EtOH:H2O. You could also reach approximately 76 mM using a 50:50 EtOH:H2O at pH ~7.5. As Michael said, Ampicillin sodium salt is quite soluble in water, yet Ampicillin Anhydrous is not.

How do you dilute ampicillin powder?

How do you dilute ampicillin sodium salt?

Ampicillin available from Sigma A-9518 (Ampicillin sodium salt), FW 371.39. To make 100ml of 100 mg/ml stock solution, dissolve 10 g of ampicillin in 50 ml of water and 50 ml of 100% ethanol.

How do you mix ampicillin 500mg?

How do you dilute ampicillin 250 mg?

While Ampicillin for Injection, USP, 1 g and 2 g, are primarily for intravenous use, they may be administered intramusculary when the 250 mg or 500 mg vials are unavailable. In such instances, dissolve in 3.5 or 6.8 mL Sterile Water for Injection, USP, or Bacteriostatic Water for Injection, USP, respectively.

How do you dissolve ampicillin powder?

I usually pour the Amp powder on the water. It dissolve very well. pouring water on the powder makes it unsoluble. and also I have seen some protocols using half water and half Et-OH.

How do I prepare ampicillin for stock?

4.0 Procedure 4.1 Ampicillin should be weighed out and added to 20mL ddiH20. 4.2 Allow for the Ampicillin to dissolve completely. 4.3 Filter Sterilize solution using a .22um filter. 4.4 Aliquot into 20 1mL eppendorf tubes. 4.5 Final Concentration for stock should be 50mg/mL. 4.6 Label and store in Ampicillin box located in Freezer #2 (026-328S-A).

How do you sterilize ampicillin?

If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H 2 O. Then pass the ampicillin solution through the washed filter. Store the ampicillin in aliquots at -20°C for 1 yr (or at 4°C for 3 mo).

How do you make sodium ampicillin?

Ampicillin (sodium salt [sodium ampicillin], m.w. = 371.40) Dissolve 1 g of sodium ampicillin in sufficient H 2 O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H 2 O. Then pass the ampicillin solution through the washed filter.

What is the final concentration of ampicillin in Selectable Media?

Note: Typical stock solutions for this antibiotic in selectable media are made at 50mg ml -1 and are used at a final concentration of 20-40 μg ml -1 for stringent plasmids and 100 μg ml -1 for relased plasmids. . Note: The preparation in ethanol allows the solution to be stored at -20°C without degrading through successive freeze-thaw cycles.