The 3xFLAG® system is an improvement upon the original system by fusing three tandem FLAG® epitopes for a total of 22 amino acids (Figure 1). Detection of fusion proteins containing 3xFLAG® is enhanced up to 200 times more than any other system.

What is 3xFLAG?

The 3xFLAG® system is an improvement upon the original system by fusing three tandem FLAG® epitopes for a total of 22 amino acids (Figure 1). Detection of fusion proteins containing 3xFLAG® is enhanced up to 200 times more than any other system.

What is the purpose of epitope tagging?

Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. Epitope tags make it possible to detect proteins when no antibody is available. This technique can be used to characterize newly discovered proteins and low abundant proteins.

How do you purify the flag tagged protein?

How to Purify FLAG-tagged Proteins? FLAG-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The FLAG-tagged protein binds to the FLAG-tag specific monoclonal antibody conjugated on an agarose gel.

What is a flagged protein?

The Flag® tag, also known as the DYKDDDDK-tag, is a popular protein tag that is commonly used in affinity chromatography and protein research for over 20 years now (6,7,8,9,10,11). As its second name suggests the tag consists of an amino acid sequence DYKDDDDK. (D=Aspartic acid; K=Lysine; Y=Tyrosine).

Why are proteins flag tagged?

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence.

What is a Myc DDK tag?

A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.

Is HA tag immunogenic?

HA tag comes from human influenza hemagglutinin (HA) corresponding to amino acids 98–106 and is a strong immunoreactive epitope making it popular to isolate, purify, detect, and track the protein of interest [40, 41].

What are flag tags used for?

Typically the Flag® tag is used for protein purification from mammalian expression systems or general immunostaining and immunoprecipitation assays.

What is an anti FLAG antibody?

General description. Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site.

What is an antibody titer?

An antibody titer is a type of blood test that determines the presence and level (titer) of antibodies in the blood. This test is carried out to investigate if there is an immune reaction triggered by foreign invaders (antigens) in the body. Image Credit: Shidlovski/Shutterstock.com What is the purpose of an antibody titer?

What is Ana titer blood test?

ANA Titer Blood Test Results Fully Explained. The ANA (antinuclear antibody) titer blood test is used to measure the amount of ANA that happens to be in the blood. This is used to determine the possibility of an autoimmune disease being present. These antibodies are proteins that are only made when the body initiates an immune response.

What are the side effects of antibody titer?

Antibody titer is a simple procedure that does not require any special preparation. While drawing the blood, a person may experience some level of discomfort ranging from stinging sensation to moderate pain. Slight bruising may also occur sometimes, which usually disappears easily.

What does a ratio of 1 200 mean in a titer?

For example, a ratio of 1:200 indicates that diluting one part of the blood sample to 200 parts of the diluent solution (saline) finally leads to an undetectable antibody level in the blood sample. A successful antibody titer result depends on the type of antibody being detected.