EdU Cell Proliferation Assay Principle. Cells grown in the presence of 5-EdU incorporate the compound at thymidine bases during S-phase. Fluorophore-labeled azide reacts with the incorporated EdU to allow detection by microscopy or flow cytometry.

What is EdU incorporation assay?

EdU Cell Proliferation Assay Principle. Cells grown in the presence of 5-EdU incorporate the compound at thymidine bases during S-phase. Fluorophore-labeled azide reacts with the incorporated EdU to allow detection by microscopy or flow cytometry.

What is tritiated thymidine?

(trĭt′ē-āt″ĭd thī′mĭ-dēn″, trĭsh′) 3. H-Tdr; a radioactively labeled nucleoside used to measure T lymphocyte proliferation in vitro. Thymidine is essential for DNA synthesis; thus the amount of 3H-Tdr taken up is a general measure of the number of new lymphocytes produced.

What is 3H-thymidine used for?

Abstract. Metabolic incorporation of 3H-thymidine into cellular DNA is a widely used protocol to monitor rates of DNA synthesis and cell proliferation. However, this radiochemical has also been reported to induce cell-cycle arrest and apoptosis in addition to DNA damage.

How is bromodeoxyuridine BrdU incorporated in cells?

Abstract. Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.

What is an incorporation assay?

Assay Description The thymidine incorporation assay, the most common assay, utilizes a strategy wherein a radioactive nucleoside, 3H-thymidine, is incorporated into new strands of chromosomal DNA during mitotic cell division.

What is the difference between EdU and BrdU?

EdU assay. Unlike assays using BrdU staining, Click-iT EdU assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside.

What is thymidine dinucleotide?

Thymidine dinucleotide (pTpT) stimulates melanogenesis in mammalian pigment cells and intact skin, mimicking the effects of UV irradiation and UV-mimetic DNA damage. Here it is shown that, in addition to tanning, pTpT induces a second photoprotective response, enhanced repair of UV-induced DNA damage.

What is the difference between thymine and thymidine?

The key difference between thymine and thymidine is that thymine is a nucleobase, whereas thymidine is a nucleoside. The term thymine and thymidine occur in biochemistry and organic chemistry as structures related to nucleic acids. Nucleic acid such as DNA and RNA are composed of nucleotides.

What is BrdU used for?

Abstract. Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation.

Why is 14c thymidine used?

Therefore, radioisotope labeling such as carbon-14 thymidine (14C-thymidine) which incorporates into new DNA, paired with a liquid scintillator counter (LSC) is commonly employed to measure the radioactivity of 14C labeled cells for long-term tracking and quantitative analysis11,12.

What is a thymidine incorporation assay?

Assay Description. The thymidine incorporation assay, the most common assay, utilizes a strategy wherein a radioactive nucleoside, 3H-thymidine, is incorporated into new strands of chromosomal DNA during mitotic cell division.

What is the meaning of tritiated thymidine?

trit·i·at·ed thy·mi·dine thymidine containing the hydrogen α-emitting radionuclide, tritium (3H or hydrogen-3); used as a marker to measure and localize by radioautography the synthesis of DNA, into which it is incorporated. Farlex Partner Medical Dictionary © Farlex 2012 tritiated thymidine (trĭt′ē-āt″ĭd thī′mĭ-dēn″, trĭsh′)

Does thymidine inhibit DNA synthesis in P388 leukemia?

Thymidine incorporation assay indicated that DNA synthesis was inhibited in the P388 leukemia cells by SEE at concentration 4.2μg/ml, which is lower than IC50; Vandana Patravale, Ratnesh Jain, in Nanoparticulate Drug Delivery, 2012

What is the role of thymidine in DNA synthesis?

Thymidine is essential for DNA synthesis; thus the amount of 3H-Tdr taken up is a general measure of the number of new lymphocytes produced. Medical Dictionary, © 2009 Farlex and Partners