What is transfection and how does it work?

Transfection is a process by which foreign nucleic acids are delivered into a eukaryotic cell to modify the host cell’s genetic makeup ( Kim & Eberwine, 2010; Chow et al., 2016 ).

How are mimcd3 cells grown in Matrigel?

Briefly, mIMCD3 cells are transfected and subsequently passaged to a Matrigel mixture, which is seeded in chamber slides and covered in growth medium. Once the spheroids are formed, Matrigel is dissolved and immunocytochemistry is performed in the chamber slides.

Is viral transduction the best way to transfect primary cells?

As compared to non-viral transfection, viral transduction is widely recognized as a highly effective method to transfect difficult-to-transfect cells such as primary cells ( Mali, 2013; Wang, Shang & Li, 2015 ).

What are the non-viral based transfection methods?

Non-viral based transfection approach can be further classified into a physical/mechanical method and chemical method. Commonly used physical/mechanical transfection method includes electroporation, sonoporation, magnetofection, gene microinjection and laser irradiation (Du et al., 2018; Hamann, Nguyen & Pannier, 2019; Meng et al., 2019).

What is the best non-viral transfection technique?

Magnetic-assisted transfection or magnetofection is another non-viral physical transfection approach and it can be used to deliver either metal-coated nucleic acids complex or magnetic-conjugated AAV vector into the host cell ( Mykhaylyk et al., 2007 ).

What are the different types of transfection protocols?

Different transfection protocols that can be divided into viral-based, non-viral based or combination of both (hybrid). Viral-based transfection, or more specifically known as transduction, involves using a viral vector to carry a specific nucleic acid sequence into a host cell.